Measurement of yeast viability mortality in the presence of chromium(VI)

The aim of this work was to develop a mathematical model - a set of equatio ns for studying the effect of Cr(VI) on yeast viability/mortality The model was built with parameters and a two-stream approach has been developed whi ch includes four parameters that indicate viability like population conditi on (PC), cell viability in the buffer (V-B), cell viability in the buffer s upplemented with the effector (V-EB), specific cell viability (V-S), and th ree parameters that indicate mortality as follows: cell mortality in the bu ffer (M-B), cell mortality in the buffer supplemented with the effector (M- EB) and specific cell mortality (M-S). The parameters were calculated on th e basis of primary results [the total cell number per mL (N-T) and the numb er of viable cells per mt (N-CFU)]. The total cell number was obtained micr oscopically with a hemacytometer and yeast viability was determined with th e colony count method (spread plate method).