A population study on two new short tandem repeat (STR) loci D6S477 and D19
S433 was performed on 214 unrelated Italian Caucasians. The DNA was amplifi
ed by PCR and separation and detection of the amplified STR fragments were
carried out by use of a PE/ABD PRISM(TM) 377 DNA sequencer 377 automated sy
stem (Applied Biosystems Division/Perkin Elmer). Both loci meet Hardy-Weinb
erg expectations. There is no evidence for departures from expectations bet
ween the two loci. The combined probability of discrimination and probabili
ty of exclusion for the two STR loci are 0.997161 and 0.883183, respectivel
y. The results demonstrate that these loci can be useful for human identifi
cation in forensic cases in Italy. (C) 1999 Elsevier Science Ireland Ltd. A
ll rights reserved.