Adaptation of human fibroblasts to radiation alters biases in DNA repair at the chromosomal level

Purpose: To determine whether adaptation to ionizing radiation biases repai r of radiation-induced chromosomal breaks. Materials and methods: Normal human fibroblasts were radiation-adapted by e xposure to 10 cGy of gamma-radiation. FISH probes for chromosomes 2, 4, 7, 18 and 19 were used to determine the chromosomal origin of the DNA in micro nuclei resulting from a subsequent 4-Gy exposure of these cells, and corres ponding non-adapted cells. Results: Compared with 4 Gy exposed but non-adapted cells, the radiation-ad apted cells subsequently exposed to 4 Gy showed an overall decrease in the frequency of micronuclei. However, the micronuclei that did form in the ada pted cells had a decreased frequency of DNA originating from chromosomes 2 and 18, an increased frequency of DNA from chromosome 19 and no change in f requency of DNA from chromosomes 4 and 7. Conclusions: Adaptation to radiation increased the overall cellular repair of radiation-induced chromosomal breaks, but also created a repair bias suc h that some chromosomes were preferentially repaired or discriminated again st, while the repair of others was unbiased.