B. Labat et al., Interaction of a plasma-sprayed hydroxyapatite coating in contact with human osteoblasts and culture medium, J BIOMED MR, 46(3), 1999, pp. 331-336
The loss of calcium from plasma-sprayed calcium phosphate ceramics (CPCs) o
n bioinert metal substrate (Ti-6A1-4V) immersed in cell culture medium with
or without human osteoblast culture was measured. The ceramics were a CPC
and a duplex system composed of a CPC layer on an alumina coating. The diss
olution of calcium compounds was monitored by measuring the calcium leaked
from the coatings into the culture medium in 15 days. Calcium was measured
by flame photometry. The surfaces of the ceramics exposed to the culture me
dium and in contact with osteoblasts were analysed by X-ray diffraction (XR
D). The dissolution process occurred in the first 6 days of contact, but th
e calcium released into the culture medium was only a small fraction of the
calcium content of the coatings. The presence or absence of osteoblasts on
the surface of the ceramics did not make significant difference for the ca
lcium release. The XRD spectra of the ceramics before and after immersion a
nd in contact with cells did not show a significant change in the compounds
of the coatings. (C) 1999 John Wiley & Sons, Inc. J Biomed Mater Res, 46,
331-336, 1999.