Expression of a carrot invertase gene in tobacco suspension cells cultivated in batch and continuous culture conditions

Authors
Des Molles, DV Gomord, V Bastin, M Faye, L Courtois, D
Citation
Dv. Des Molles et al., Expression of a carrot invertase gene in tobacco suspension cells cultivated in batch and continuous culture conditions, J BIOSCI BI, 87(3), 1999, pp. 302-306
Citations number
32
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN journal
13891723 → ACNP
Volume
87
Issue
3
Year of publication
1999
Pages
302 - 306
Database
ISI
SICI code
1389-1723(199903)87:3<302:EOACIG>2.0.ZU;2-O
Abstract
Plant cells (Nicotiana tabacum) were genetically modified to produce an het erologous protein, the acidic invertase from carrot, and invertase producti on from suspension tobacco cells was investigated. Suspension cultures were grown in shake flasks and stirred bioreactor. Total invertase activity was growth related. A 75 d continuous culture in 10 l bioreactor was performed . Our study demonstrates the high potential of plant cell cultures for long term production of heterologous protein.