Optimization of n variable biological experiments by evolutionary operation-factorial design technique

Citation
R. Tunga et al., Optimization of n variable biological experiments by evolutionary operation-factorial design technique, J BIOSCI BI, 87(2), 1999, pp. 224-230
Citations number
22
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN journal
13891723 → ACNP
Volume
87
Issue
2
Year of publication
1999
Pages
224 - 230
Database
ISI
SICI code
1389-1723(199902)87:2<224:OONVBE>2.0.ZU;2-S
Abstract
Since the traditional approach to optimization of biological systems based on the single variable search technique is incapable of detecting the true optimum when a number of chemicals are used together as inducers, the Evolu tionary Operation (EVOP)-factorial design technique was applied successfull y to optimize the concentrations of vitamin (biotin), metal ion (CaCl2) and plant hormone (NAA) for maximizing enzyme (protease) production by Rhizopu s oryzae (RO IIT RB-13, NRRL-21498) in solid state fermentation,Addition of inducers to an optimal level (biotin-2.5 ppm, CaCl2-20 ppm and NAA-12.5 pp m) resulted in a similar to 1.9-fold increase in protease production.