Cloning and sequencing of the Pz-peptidase gene from Bacillus licheniformis N22

Authors
Akiyama, K Mori, K Takata, R
Citation
K. Akiyama et al., Cloning and sequencing of the Pz-peptidase gene from Bacillus licheniformis N22, J BIOSCI BI, 87(2), 1999, pp. 231-233
Citations number
18
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN journal
13891723 → ACNP
Volume
87
Issue
2
Year of publication
1999
Pages
231 - 233
Database
ISI
SICI code
1389-1723(199902)87:2<231:CASOTP>2.0.ZU;2-A
Abstract
Pz-peptidase is an endopeptidase that cleaves the synthetic substrate Pt-pe ptide (4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-Arg), which was origina lly developed for the assay of collagenase. The Pt-peptidase gene of Bacill us licheniformis N22 was cloned and sequenced. The gene consists of 628 ami no acids with a motif for zinc-dependent metalloprotease, and shares 42% am ino acid identity with the oligoendopeptidase of Lactococcus lactis. This i s the first report on the gene structure of a Pz-peptidase.