Photodynamic therapy: In vivo photosensitizing efficacy of bis(tri-n-hexylsiloxy)silicon phthalocyanine against achromic M6 melanocytes. ESR study ofactive oxygen intermediates

Authors
Decreau, R Viola, A Richard, MJ Jeunet, A Julliard, M
Citation
R. Decreau et al., Photodynamic therapy: In vivo photosensitizing efficacy of bis(tri-n-hexylsiloxy)silicon phthalocyanine against achromic M6 melanocytes. ESR study ofactive oxygen intermediates, J PORPHYR P, 2(4-5), 1998, pp. 405-414
Citations number
60
Categorie Soggetti
Chemistry
Journal title
JOURNAL OF PORPHYRINS AND PHTHALOCYANINES
ISSN journal
10884246 → ACNP
Volume
2
Issue
4-5
Year of publication
1998
Pages
405 - 414
Database
ISI
SICI code
1088-4246(199807/10)2:4-5<405:PTIVPE>2.0.ZU;2-I
Abstract
The in vitro photodynamic effect of bis(tri-n-hexylsiloxy)silicon phthalocy anine has been evaluated against the melanotic M6 cell line. The results sh owed that at 10(-5) M dose, LD50 is Obtained for a 150 J cm(-2) light dose and LD90 for 540 J cm(-2). Electron spin resonance spectroscopy was used wi th spin traps to study the type I and type II photochemical pathways involv ed and to detect active oxygen intermediates such as singlet oxygen, oxygen superoxide and hydroxyl radical. The two mechanisms occurred simultaneousl y and no change was observed when the phthalocyanine was entrapped in lipos omes. (C) 1998 John Wiley & Sons, Ltd.