RT-PCR amplification of various canine cytokines and so-called house-keeping genes in a species-specific macrophage cell line (DH82) and canine peripheral blood leukocytes

Total ribonucleic acid (RNA) isolated from a continuous canine macrophage c ell line (DH82) was used in reverse transcription polymerase chain reaction s (RT-PCR) for the detection of transcripts of interleukin (IL)-8, -12, and tumour necrosis factor-alpha (TNF). Three different methods of RNA isolati on (standard guanidinium-thiocyanate method with and without application of RNA matrix, and boiling) mere used and compared in regard to RT-PCR result s. The most suitable method was used to establish RT-PCR amplification of m RNA transcripts of IL-2, -10, and interferon-gamma (IFN) in RNA isolated fr om canine peripheral blood leukocytes. Integrity; of RNA isolates was ensur ed by amplification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) or beta-actin. IL-8, -12, and TNF were amplified from RNA isolated by various methods. Use of guanidinium-thiocyanate with and without RNA matrix gave th e most consistent results. Boiling as a mean of RNA isolation was quick and easy, but the RT-PCR results were extremely variable and multiple smaller bands were observed in the agarose gel in some preparations. IL-2, -10 and IFN transcripts were amplified from RNA isolated with guamidimium-thiocyana te from leukocytes stimulated with concanavalin A. DNase-treatment of RNA i solates was necessary to assure the destruction of genomic DNA and to avoid amplification of genomic sequences. This was especially a problem when usi ng primers for GAPDH, beta-actin, IL-12, and TNF. Lack of DNase-treatment m ay lead to false positive results. This map be especially a problem when am plification of so-called house-keeping genes is used as internal control fo r RNA integrity. These findings demonstrated that isolation of total RNA wi th guanidinium-thiocyanate followed by DNase-treatment gave reliable and co nsistent results for detection of cytokine transcripts by RT-PCR in a canin e macrophage cell line and canine peripheral blood leukocytes.