The pBuM189 satellite DNA family was found to be species specific for Droso
phila buzzatii. It consists of slightly AT-rich tandemly arranged repeats w
ith a high copy number in the genome and shows a very high level of intrasp
ecific sequence similarity. pBuM189 repeats cannot be detected in the genom
es of closely related species such as Drosophila serido, Drosophila borbore
ma and Drosophila koepferae. The data support the marginal systematic posit
ion of D. buzzatii within the buzzatii cluster of the Drosophila repleta gr
oup.