Selective expression of serotonin receptor transcripts in the mammalian cochlea and its subdivisions

Citation
Ck. Oh et al., Selective expression of serotonin receptor transcripts in the mammalian cochlea and its subdivisions, MOL BRAIN R, 70(1), 1999, pp. 135-140
Citations number
25
Categorie Soggetti
Neurosciences & Behavoir
Journal title
MOLECULAR BRAIN RESEARCH
ISSN journal
0169328X → ACNP
Volume
70
Issue
1
Year of publication
1999
Pages
135 - 140
Database
ISI
SICI code
0169-328X(19990618)70:1<135:SEOSRT>2.0.ZU;2-6
Abstract
Expression of serotonin receptor (5-HTR) mRNA has been determined in the ma mmalian cochlea and its subdivisions by reverse transcriptase-polymerase ch ain reaction (RT-PCR) analysis. Specific primers targeting individual 5-HTR s 1-7 directed amplification of 5-HTR subtypes 1A, 1B, 2B, 2C, 3, 5B, and 6 from mouse cochlea cDNA. No evidence of expression was obtained for 5-HTRs 1D, 2A, 4 (L and S), 5A, and 7. The distribution of receptor mRNA within t he cochlea was determined with application of RT-PCR to morphologically def ined microdissected subfractions of the rat cochlea. Messages for 5-HTR sub types 1A, 1B, 2B, and 6 were present in the organ of Corti, lateral wall, a nd spiral ganglion subfractions. Messages for 5-HTR subtypes 2C, 3 and 5B w ere found in the spiral ganglion, but not in the organ of Corti or lateral wall fractions. The existence of transcripts for 5-HTRs 1A, IB, 2B and 6 in the organ of Corti is consistent with a role for these receptors in seroto nin-mediated modulation of the mechanosensory signal. (C) 1999 Elsevier Sci ence B.V. All rights reserved.