Alcohol-soluble endosperm proteins (prolamins) from some cereals (e.g. whea
t, barley, and rye) give origin upon proteolytic digestion to biologically-
active antinutritional peptides able to adversely affect in vivo the intest
inal mucosa of coeliac patients, whereas prolamins from other cereals (e.g.
maize and rice) do not. These antinutritional peptides are also able to: (
a) prevent in vitro recovery of atrophic coeliac mucosa; (b) to inhibit dif
ferentiation of isolated rat fetal and chick fetal intestines, and (c) to i
nteract with undifferentiated cells either agglutinating them or affecting
their proliferation and metabolism. Studies performed with A-gliadin, a hig
hly purified bread wheat prolamin fraction, and its fragments obtained eith
er by chemical cleavage of A-gliadin or by synthesis from aminoacids, clear
ly pointed out to a few small sequences very rich in glutamine and proline
residues as the biologically-active agents. Several protective substances,
including mannan and N.N',N "-triacetylchitotriose, have been identified as
being able to prevent the effects of these peptides in vitro, but the evid
ence of their in vivo activity is still missing. The present paper provides
a synthetic overview of the available data concerning this highly complex
matter and offers a critical appraisal of present hypotheses on the action
mechanism of biologically-active peptides derived from cereal prolamins.