Identification of a novel Sry-related gene and its germ cell-specific expression

Citation
E. Osaki et al., Identification of a novel Sry-related gene and its germ cell-specific expression, NUCL ACID R, 27(12), 1999, pp. 2503-2510
Citations number
51
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEIC ACIDS RESEARCH
ISSN journal
03051048 → ACNP
Volume
27
Issue
12
Year of publication
1999
Pages
2503 - 2510
Database
ISI
SICI code
0305-1048(19990615)27:12<2503:IOANSG>2.0.ZU;2-9
Abstract
Sox family proteins are characterized by a unique DNA-binding domain, a HMG box which shows at least 50% sequence similarity with mouse Sry, the sex-d etermining factor. At present almost 30 Sox genes have been identified, Mem bers of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human disea ses, including sex reversal. Here we report the isolation of a novel member of the Sox gene family, Sox30, which may constitute a distinct subgroup of this family, Using a bacterially expressed DNA-binding domain of Sox30, we show that it is able to specifically recognize the ACAAT motif. Furthermor e, Sox30 is capable of activating transcription from a synthetic promoter c ontaining the ACAAT motif. The specific expression of Sox30 in normal teste s, but not in maturing germ cell-deficient testes, suggests the involvement of Sox30 in differentiation of male germ cells. Mapping analyses revealed that the Sox30 gene is located on human chromosome 5 (5q33) and on mouse ch romosome 11.