Irradiation of amelanotic melanoma cells with 532 nm high peak power pulsed laser radiation in the presence of the photothermal sensitizer Cu(II)-hematoporphyrin: A new approach to cell photoinactivation

Cu(II)-hematoporphyrin (CuHp) was efficiently accumulated by B78H1 amelanot ic melanoma cells upon incubation with porphyrin concentrations up to 52 mu M When the cells incubated for 18 h with 13 mu M CuHp were irradiated with 532 nm light from a Q-switched Nd: YAG laser operated in a pulsed mode (10 ns pulses, 10 Hz) a significant decrease in cell survival was observed. Th e cell photoinactivation was not the consequence of a photodynamic process, as CuHp gave no detectable triplet signal upon laser hash photolysis excit ation and no decrease in cell survival was observed upon continuous wave ir radiation. Thus, it is likely that CuHp sensitization takes place by photot hermal pathways. The efficiency of the photoprocess was modulated by differ ent parameters; thus, while varying the amount of added CuHp in the 3.25-26 mu M range had little effect, pulse energies larger than 50 mJ and irradia tion times of at least 120 s were necessary to induce a cell inactivation o f about 50%, The porphyrin-cell incubation time prior to irradiation had a major influence on cell survival, suggesting that the nature of the CuHp mi croenvironment can control the efficiency of photothermal sensitization.