L. Cistue et al., Influence of anther pretreatment and culture medium composition on the production of barley doubled haploids from model and low responding cultivars, PL CELL TIS, 55(3), 1998, pp. 159-166
Different pretreatments were given to anthers of barley before culturing, a
nd their effects assessed on the frequency of embryos and green doubled hap
loid plants produced. Mannitol pretreatment was better than cold pretreatme
nt for some low responding cultivars. Optimal concentration of mannitol for
pretreatment depended on cultivar. Low responding genotypes needed a highe
r concentration of mannitol than responsive ones. The addition of Ficoll to
liquid medium increased the number of embryos and green plants. The influe
nce of the growth regulators 2,4-D and TIBA was assayed using ten cultivars
of barley grown in Spain. The anti-auxin TIBA gave good embryo production
with some of the low responding cultivars. Two row-type cultivars always pr
oduced higher number of embryos and green plantlets than six row-type. The
application of these modifications to 10 F-1 hybrids with potential agronom
ic value, allowed the production of almost 1000 doubled haploid plants from
only 3500 anthers. Up to two doubled haploid plants per flower were produc
ed from the cross Monlon x Sonja.