Two steinemematid species, Steinemema kraussei and Steinernema sp. E1, whic
h were not reported during previous surveys in Belgium, were recovered from
woodland soils by direct extraction of infective stages from soil samples.
Identification of the juveniles was based on their morphology and confirme
d by RFLP analysis of the PCR amplified ITS-rDNA region. Baiting by Galleri
a mellonella larvae of soil samples in which these two species were present
, did not result in the normal infection development and was not followed b
y juvenile migration. It is concluded that the presence of some populations
of these two species in soil may not be revealed by Galleria baiting.