In a sequencing batch reactor (SBR) granules of aerobic heterotrophic micro
organisms were cultured. The effect of different operational conditions on
the formation of these aerobic granules were studied. The time allowed for
settling was the main parameter to select for growth of bacteria in well se
ttling granules. Both a short HRT and a relative high shear were found favo
rable for granulation. A substrate loading rate of 7.5 kg COD/(m(3) day) wa
s applied. This Ted to formation of granules with an average diameter of 3.
3 mm and a biomass density of 11.9 gVSS/l(granule). Based on microscopic ob
servations a hypothesis for the granulation process was formulated. The rea
ctor was started up without any carrier material present. At the beginning
filamentous fungal pallets dominated the reactor. These pellets functioned
as an immobilization matrix in which bacteria could grow out to colonies. A
fter a certain rime the fungal pellets fell apart due to lysis in the inner
parr of the pellets, the bacterial colonies could now remain in the reacto
r because they were large enough to settle sufficiently Fast. These colonie
s further grew out to granules. This paper shows that granule formation in
aerobic reactors is feasible and can be exploited to increase the volumetri
c conversion capacity of such reactors. (C) 1999 Elsevier Science Ltd. All
rights reserved.