Valproic acid poisoning: Urinary analysis using H-1 NMR and GC MS

Analysis of urine samples from two poisoned patients was performed using pr oton nuclear magnetic resonance (H-1 NMR) spectroscopy and a classical GC/M S protocol. Valproic acid as its glucuronide was identified and quantified in the H-1 NMR spectra recorded directly from 0.5 mL of the collected sampl es. Moreover, a significant lactic aciduria could be observed. Comparison w ith the GC/MS findings suggests that H-1 NMR spectroscopy can be clinically useful.