Exclusion of HIV coreceptors CXCR4, CCR5, and CCR3 from the HIV envelope

Both HIV-1 primary isolates and laboratory strains incorporate cell-derived molecules into their envelopes depending on the host cell in which they ar e grown, This incorporation is not random and, specifically, HIV-1 has been shown to select against the incorporation into its surface of CD4, its mai n receptor, In this study, we have looked at the incorporation of HIV corec eptors CXCR4, CCR5, and CCR3 into the HIV envelope. For this purpose, we gr ew HIV-1 primary isolate BZ167 in several cell lines and PBMCs, and the env elope profiles of the resulting viruses were determined with a virus-bindin g ELISA, While the virus particle gained several molecules when passed thro ugh the different cell lines (e.g., ICAM-3, LFA-1, ICAM-1, or MHC class II) , BZ167 never incorporated significant levels of CXCR4, CCR5, or CCR3 into its envelope even though some or all of the cell lines in which it was grow n expressed them. These results show that HIV-1 selects against the incorpo ration of these chemokine receptors into its envelope molecule, as it does against the incorporation of CD4.