Strain-dependent induction of allergic rhinitis without adjuvant in mice

Citation
M. Okano et al., Strain-dependent induction of allergic rhinitis without adjuvant in mice, ALLERGY, 54(6), 1999, pp. 593-601
Citations number
53
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Immunology
Journal title
ALLERGY
ISSN journal
01054538 → ACNP
Volume
54
Issue
6
Year of publication
1999
Pages
593 - 601
Database
ISI
SICI code
0105-4538(199906)54:6<593:SIOARW>2.0.ZU;2-J
Abstract
Background: To date, no murine models have been reported to show the induct ion of both antigen-specific IgE and nasal eosinophilia, two of the major h allmarks of allergic rhinitis, after local sensitization in the absence of adjuvants, a phenomenon which reflects natural exposure. In this report, we attempted to establish a murine model representing an initiation of allerg ic rhinitis. Methods: BALB/c, CBA/J, and C57BL/6 mice were sensitized intranasally to Sc histosoma mansoni egg antigen (SEA) solely. After repeated sensitization, s erum Ab titers, nasal eosinophilia, and cytokine production by nasal lympho cytes were determined. Results: BALB/c mice produced SEA-specific IgE after repeated sensitization . High-dose sensitization to SEA induced IgE production in CBA/J mice, whil e C57BL/6 mice did not show the production throughout the period observed, suggesting that IgE production was regulated genetically. BALB/c mice also exhibited nasal eosinophilia after the nasal challenge. In addition, nasal lymphocytes sensitized with SEA intranasally produced significant amount of IL-5 in vitro. Conclusions: These results suggest that intranasal sensitization with SEA i n the absence of adjuvants induces a Th2 immune reaction, reflecting the ha llmarks of the initiation of allergic rhinitis both in vivo and in vitro, w hich is genetically regulated.