A mixed solids waste (MSW) feedstock, comprising construction lumber waste
(35% oven-dry basis), almond tree prunings (20%), wheat straw (20%), office
waste paper (12.5%), and newsprint (12.5%), was converted to ethanol via d
ilute-acid pretreatment followed by enzymatic hydrolysis and yeast fermenta
tion. The MSW was pretreated with dilute sulfuric acid (0.4% w/w) at 210 de
grees C for 3 min in a 4-L steam explosion reactor, then washed with water
to recover the solubilized hemicellulose. The digestibility of water-washed
, pretreated MSW was 90% in batch enzymatic hydrolysis at 66 FPU/g cellulos
e. Using an enzyme-recycle bioreactor system, greater than 90% cellulose hy
drolysis was achieved at a net enzyme loading of about 10 FPU/g cellulose.
Enzyme recycling using membrane filtration and a fed-batch fermentation tec
hnique is a promising option for significantly reducing the cost of enzyme
in cellulose hydrolysis. The hexose sugars were readily fermentable using a
Saccharomyces cerevisiae yeast strain that was adapted to the hydrolysate.
Solid residue after enzyme digestion was subjected to various furnace expe
riments designed to assess the fouling and slagging characteristics. Result
s of these analyses suggest the residue to be of a low to moderate slagging
and fouling type if burned by itself.