Lignin peroxidase production by Streptomyces viridosporus T7A - Use of corn oil as a carbon source

Lignin peroxidase (LiP) production cost should be reduced to justify its us e in the control of environmental pollution. In this work, we studied the e nzyme production by Streptomyces viridosporus T7A using glucose or corn oil as a carbon source having 0.65% yeast extract as a nitrogen source. Enzyme activity, observed using either 0.65% glucose or corn oil at 0.1, 0.5, and 1.0% concentration, was 300, 150, 300, and 200 U/L, respectively. Although higher enzyme activity was obtained in both media containing 0.65% glucose and 0.5% corn oil, the use of corn oil resulted in a better LiP stability. When combined carbon sources were used, higher values of enzyme activity ( 360, 350, and 225 U/L) were observed in media with 0.65% glucose and supple mented with 0.1, 0.5, and 1.0% corn oil, respectively. Although the presenc e of both glucose and 0.5% corn oil is favorable for LiP production, satisf actory results in terms of enzyme production and stability could be also ob served using 0.5% corn oil as a sole carbon source, which may lead to reduc ed production costs of the LiP enzyme.