Novel use of a static modification of two-dimensional correlation analysis. Part II: Hetero-spectral correlations of protein Raman, FT-IR, and circular dichroism spectra

The two-dimensional (2D) static correlation model developed in the previous paper is here extended to hetero-spectral applications for the correlation of spectral features arising from two separate techniques. The calculation al method is detailed, and examples are given of Raman vs. circular dichroi sm (CD) (electronic) and Raman vs. Fourier transform infrared (FT-IR) 2D co rrelation and disrelation maps with fractional secondary structures used as the perturbation. This approach allows use of well-established secondary s tructure assignments of spectral features in one technique to interpret str ucturally less clearly assigned bands, particularly for beta-turns, or poss ibly misassigned bands in other spectra via their calculated 2D correlation s.