DETERMINATION OF HISTAMINE BY CAPILLARY ZONE ELECTROPHORESIS USING A LOW PH PHOSPHATE BUFFER - APPLICATION IN THE ANALYSIS OF FISH AND MARINE PRODUCTS

Histamine levels in samples of fish and marine products were determine d by capillary zone electrophoresis using phosphate buffer at pH 2.44 and UV detection at 214 nm. A plot of the area of the peaks versus his tamine concentration was linear over the range of 1-100 ppm with a cor relation coefficient of 0.9996. Calibration using the height of the pe ak also gave good correlation, with a correlation coefficient of 0.996 9, at concentrations between 1 ppm and 20 ppm. The recovery of added h istamine in different samples of fish and fish products was, on averag e, 99.65%. The method described in this work was used for the quantita tion of histamine in fish and fish products.