Nucleotide sequences of genes for ribosomal protein L41 and tRNA(Thr)(AGU)from Coprinus cinereus

The nucleotide sequences of genes for the homolog in Coprinus cinereus of t he eukaryotic ribosomal protein L41 and for tRNA(Thr)(AGU) are reported. Th e gene for tRNA(Thr)(AGU) was located upstream of the gene for the L41 ribo somal protein, and these genes were adjacent to each other but in opposite orientations. The deduced amino acid sequence of ribosomal protein L41 exhi bited strong homology to those of L41 proteins of several yeasts. The 56th amino acid of the deduced protein was proline, as it is in the L41 protein of a cycloheximide-sensitive strain of yeast. The putative secondary struct ure of the tRNA gene resembled the characteristic cloverleaf structure of t RNAs. Elements resembling an A-box and a B-box were found in the gene for t RNA(Thr)(AGU). These boxes are known as internal promoter elements in genes for eukaryotic tRNAs, (C) 1999 Elsevier Science B.V. All rights reserved.