An improved micro-method for obtaining chromosome preparations from individual haemopoietic colonies

Authors
Kadhim, MA Pocock, DA Lorimore, SA Wright, EG
Citation
Ma. Kadhim et al., An improved micro-method for obtaining chromosome preparations from individual haemopoietic colonies, BR J HAEM, 105(3), 1999, pp. 673-675
Citations number
11
Categorie Soggetti
Hematology,"Cardiovascular & Hematology Research
Journal title
BRITISH JOURNAL OF HAEMATOLOGY
ISSN journal
00071048 → ACNP
Volume
105
Issue
3
Year of publication
1999
Pages
673 - 675
Database
ISI
SICI code
0007-1048(199906)105:3<673:AIMFOC>2.0.ZU;2-6
Abstract
A robust method for obtaining chromosome preparations from individual haemo poietic colonies in semisolid media is described. The accumulation of metap hases and the hypotonic treatment of cells were carried out in the culture dish and individual colonies were transferred onto poly-L-lysine-treated sl ides and fixed stepwise prior to staining. The technique produced high yiel ds of well-spread metaphases facilitating clonal cytogenetic analysis.