Establishment of a near-triploid human B-cell lymphoma cell line with t(14;18) and a p53 gene point mutation

We report a rare large B-cell non-Hodgkin's lymphoma having a characteristi c near-triploid cell population with add(17)(p22) and t(14;18)(q32;q21) tra nslocation. We also established and characterized a new cell line (Tli cell ) derived from the present lymphoma. A codon 180 mutation (GAG-->GAT) in th e p53 gene was detected, t(14;18)(q32:q21) was revealed juxtaposition of th e bcl-2 and JH genes. Immunoprecipitation analyses of p53 and bcl-2 reveale d that abnormality of the p53 protein and aberrant bcl-2 expression, which may protect cells from apoptosis, may be critical to the development of leu kaemogenesis exhibiting near-triploid chromosomes.