Treatment of medullary thyroid carcinoma by combined expression of suicideand interleukin-2 genes

Inherited medullary thyroid carcinomas (MTC) are aggressive and resistant t o conventional chemo- and radiotherapies. We evaluated a novel strategy for treatment of MTC, combining "suicide" and interleukin-2 (IL-2) gene therap ies. Tumors were produced in Wag/Rij rats by orthotopic injection of the rM TC 6-23 cell line, and/or derivatives expressing the herpes simplex virus 1 thymidine kinase (HSV1-TK) gene (rMTC-TK). Ganciclovir, a nucleoside analo g selectively transformed to a toxic metabolite by HSV1-TK, totally eradica ted rMTC-TK tumors in 60% of the animals. 1:1 rMTC and rMTC-TK mixed tumors were also strongly inhibited by ganciclovir (P < 0.05), indicating the occ urrence of an efficient "bystander" effect in vivo. Double labelling of rMT C cell membranes and apoptotic nuclei revealed that, as with the TK+ cells, some TK- cells died by apoptosis. A 1:1 mixture of rMTC and rMTC-TK cells was administered to produce established tumors and then rMTC cells, transfe cted to express the IL-2 gene (rMTC-IL2), were inoculated. Combined gancicl ovir and IL-2 treatment improved the inhibition of tumor growth compared to that following ganciclovir alone (86% compared to 54%, P < 0.05). This tre atment also significantly enhanced macrophage activation and tumor infiltra tion by CD8(+) and CD4(+) T lymphocytes. These results open an avenue for c ombining suicide and immunoregulatory gene therapies for MTC management in man.