Uptake of glucose analogues by colonic tumour cells during growth and after treatment with hydroxyurea

SW620 cells were grown in tissue culture Basks to various cell densities pr oducing populations of cells with a range of proliferative indices. The upt ake of the two glucose analogues, deoxy-D-glucose (DG) and 3-O-methylglucos e (OMG) was determined and found to be associated with S-phase fraction. Th e strong correlation between DG and OMG uptakes suggested that proliferatio n-related changes in transmembrane transport accounted for the association with S-phase fraction. Treatment of SW620 cells with the cell cycle inhibit or hydroxyurea was found to increase the uptake of DG and OMG in a time-dep endent manner. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.