Isolation and identification of a novel STR (D17S2204) in the vicinity of NF1 locus

Using a synthetic oligonucleotide (dA-dC)(15) as a probe to screen a probe pool made by microdissected human chromosome band 17q11-q12, a DNA fragment containing (CA)(16) was isolated, which is a novel short tandem repeat (ST R) determined by searching in the GenBank and GDB, It has 8 allelic types w ith a PIC value of 0.61. The novel STR is conformed with Mendelian segregat ion according to the linkage analysis of a three-generation neurofibromatos is type I (NF1) pedigree. The STR was localized at chromosome band 17q11-q1 2 in the vicinity of NF1 gene by FISH analysis. The accession number of the STR in GenBank and GDB is G32112 and D17S2204 respectively.