Enantioseparation of tropa alkaloids by means of anionic cyclodextrin-modified capillary electrophoresis

Anionic cyclodextrins (CDs), i.e., sulfated CD, sulfobutylether beta-CD (SB E-beta-CD) and heptakis(2,3-di-O-acetyl-6-sulfato)-beta-CD (HDAS-beta-CD), were used to separate the isomers of atropine, homatropine, ipratropium, sc opolamine and butylscopolamine. Variations in the pH value and the concentr ation of the various CDs revealed the sulfated beta-CD to be superior to th e other derivatives. In a basic medium at low concentrations of sulfated CD the enantiomers of atropine, homatropine and ipratropium were well resolve d in a short migration time, which is appropriate for the routine analysis of the enantiomeric excess. Scopolamine and butylscopolamine compounds coul d not be separated with either CD.