Formation of N-(2-hydroxyethyl)valine due to exposure to ethylene oxide via tobacco smoke: A risk factor for onset of cancer

Human exposure to ethylene oxide (EtO) occurs mainly through inhalation of occupational polluted air and tobacco smoke. EtO is able to react with DNA and proteins producing some molecular adducts. One of these, resulting from reaction between EtO and valine in hemoglobin, is N-(2-hydroxyethyl) valin e (HOEtVal). This adduct represents a biological effective dose marker, the level of which correlates linearly with the alkylating activity occurring in DNA. The aim of the present study was to measure HOEtVal in 146 urbanize d adult and healthy subjects, nonoccupationally exposed to EtO, and to corr elate it with smoke habits. HOEtVal showed a direct positive relationship t o tobacco smoke exposure quantified by questionnaire, urinary cotinine (r = 0.64509), and the number of cigarettes (r = 0.6308) actively or passively smoked. Results relative to HOEtVal and urinary cotinine in adults distingu ish well between active and passive smokers but do not allow distinguishmen t between passive smokers and nonsmokers. Nevertheless, several authors dem onstrated a very good capacity of cotinine to discriminate inside groups of adolescents passive smokers. Therefore, the future objective of the presen t study is a closer inspection of the two biomarkers with respect to passiv e exposure to tobacco smoke considering a large group of adolescents. Final ly, the correlation between urinary cotinine and HOEtVal increases knowledg e about early steps of the carcinogenic process due to active exposure to t obacco smoke. (C) 1999 Academic Press.