This paper describes an eighty days toluene vapour removal from air by a pe
at-based biofilter to study the effect;of different inocula and nutrient su
pplies on the biofiltration efficiency. Four pure microbial strains were us
ed for the inoculation of the biofilter matrix: i.e. Arthrobacter paraffine
us, from the American Type Culture Collection (ATCC) and a further three ar
omatic hydrocarbons degraders selected from among those in private collecti
ons. The toluene inlet load was maintained at constant value throughout the
trial . To improve toluene biofiltration, changes in the inoculation of th
e filter bed have been diversified. We have also investigated supply of nut
rients by various aqueous solutions to humidify the filter packing material
. Nutrients introduced by these solutions contained inorganic and organic n
itrogen forms. To evaluate the influence of nutrient supplies, the operatin
g parameter measurements have been monitored on an 80 day cycle. Parameters
observed included the filter bed temperature, pressure drop and the toluen
e concentrations at both the entry and exit of the biofilter, the pH and ph
ysiological activities resulting from the various operational conditions.