Cell wall teichoic acids of Actinomadura viridis VKM Ac-1315T

The cell walls of Actinomadura viridis contain poly(glycosylglycerol phosph ate) chains of complex structure. On the basis of NMR spectroscopy of the p olymer and glycosides thereof the following structural units were found: be ta-D-Galp3Me-(1-->4)[beta-D-Glcp,-(1-->6)]-beta-D-Galp-(1-->1)-snGro (G1); beta-D-Galp-(1-->4)-beta-D-Galp-(1-->1)-snGro (G2); beta-D-Galp3Me-(1-->4)- beta-D-Galp-(1-->1)-snGro (G2a); beta-D-Galp-(1-->1)-snGro (G3); beta-D-Gal p-(1-->1)[beta-D-Galp-(1-->2)]-snGro (G4); beta-D-Glcp-(1-->2)-snGro (G5). Glycosides G1, G2 and G3 were the predominant components of the teichoic ac id: they formed the polymer chain via phosphodiester bonds involving C-3 of the glycerol residue and C-3 of the galactosyl residue which in turn glyco sylates C-1 of the glycerol residue. Whether the different glycosides make up the one chain or whether there are several poly(glycosylglycerol phospha te) chains in the cell wall remains to be determined. It was suggested that the minor component G5 is located at the nonterminal end of the chains. Co mpound G3 which contains disubstituted glycerol resudues (unusual for the t eichoic acid) was also found as a minor component; this may be the glycosid e of a new type of teichoic acid, or a glycoside on the terminal end of the above mentioned chains. In addition, small amounts of 1,3-poly(glycerol ph osphate) chains were found in the cell wall.