Objective: To assess the magnitude of the putative effect of atovaquone on
the pharmacokinetics of proguanil and to determine whether the pharmacokine
tics of atovaquone are affected by concomitant administration of proguanil,
with both drugs administered for 3 days to healthy adult volunteers.
Methods: This was an open-label, randomized, three-way cross-over study, in
which 18 healthy volunteers received 400 mg proguanil, 1000 mg atovaquone
and 1000 mg atovaquone + 400 mg proguanil. Each treatment was given once da
ily for 3 days with a 3-week wash-out period between each occasion. For the
assay of proguanil, cycloguanil and atovaquone, blood was sampled before d
osing and at regular intervals over 8 days when proguanil was given, and ov
er 17 days when atovaquone was given.
Results: The geometric mean of the area under the atovaquone plasma concent
ration-time curve calculated from 0 to 24 h after the last dose (AUC(0-->24
h)) was 180 mu g . ml(-1) . h following administration of atovaquone alone
and 193 mu g . ml(-1) . h following atovaquone with proguanil. The geometri
c mean AUC(0-->24h) for proguanil was 6296 ng . ml(-1) . h after proguanil
alone and 5819 ng . ml(-1) . h following proguanil with atovaquone. The cor
responding values for the metabolite cycloguanil were 1297 ng . ml(-1) . h
and 1187 ng . ml(-1) . h, respectively. The geometric mean elimination half
-life (t(1/2)) Of atovaquone was 57.1 h when given alone and 59.0 h when ad
ministered together with proguanil. The corresponding geometric mean values
of t(1/2) for proguanil were 13.7 h and 14.5 h. Exploratory statistical an
alysis showed no important gender effects on the pharmacokinetics of atovaq
uone, proguanil, or cycloguanil.
Conclusion: The pharmacokinetics of atovaquone and proguanil and its metabo
lite, cycloguanil, were not different when atovaquone and proguanil were gi
ven alone or in combination.