Deletion of 24 open reading frames from chromosome XI from Saccharomyces cerevisiae and phenotypic analysis of the deletants

As a part of the EUROFAN program, 24 open reading frames from Saccharomyces cerevisiae (YKR010c to YKR013w, YKR015c to YKR025w, YKR081c to YKR083c, YK R087c to YKR091w and YKR096w) were disrupted in two genetic backgrounds, FY 1679 and W303. Systematic deletions and phenotypic analysis were performed following a hierarchical strategy, the so-called 'mass murder'. Of the 24 g enes thus deleted, four are essential, whereas the deletion of 17 did not r eveal any significant difference between the parental and mutant strains. D eletions of the remaining three show some growth phenotype; ykr024c mutants grow slowly under any conditions, ykr019c mutants grow slower in a rich me dium and ykr082w mutants are temperature sensitive, being unable to germina te at 30 degrees C and above. (C) 1999 Elsevier Science B.V. All rights res erved.