An integrated somatic cell hybrid, YAC, and BAC map of the Rmc1 region of mouse chromosome 1

Rmc1, the cellular receptor for the polytropic class of murine retroviruses , determines the tissue tropism of the virus and therefore plays a critical role in the pathogenesis of polytropic virus-induced leukemia. Previously we reported the physical mapping of this gene to a 5-cM region of mouse chr omosome 1 and the construction of a yeast artificial chromosome (YAC) conti g across this region. In this report we describe the refinement of the Rmc1 candidate region to approximately 600 kb and the generation of an integrat ed somatic cell hybrid, YAC, and bacterial artificial chromosome contig spa nning the region. A number of genes and loci were physically ordered along the chromosome, including a recently identified candidate for Rmc1, (C) 199 9 Academic Press.