I. Bechmann et al., FasL (CD95L, Apo1L) is expressed in the normal rat and human brain: Evidence for the existence of an immunological brain barrier, GLIA, 27(1), 1999, pp. 62-74
Despite the mechanical blood-brain barrier, activated T-cells can cross bra
in vessels. Thus, the CNS is routinely surveyed by immune competent cells;
yet the healthy brain is not a target of antigen-specific immune reactions.
Therefore, mechanisms must exist to prevent brain-antigen-specific T-cells
from inducing immune responses. Data indicate that activated T-cells enter
ing the CNS may undergo apoptosis rather than leaving the brain to induce i
mmune responses. Applying RT-PCR, Western-blots, and immunocytochemistry, w
e have demonstrated expression of the apoptosis-inducing protein Fas ligand
on astrocytes and neurons of apparently normal rat and human brains. Fast-
positive astrocytes were often situated in close vicinity to cerebral blood
vessels in vivo and induced apoptosis of Fas expressing Jurkat cells in vi
tro. We propose that similar to other immune privileged organs Fast-induced
apoptosis of activated T-cells in the brain protects the tissue from self
damaging immune attacks by forming an immunological brain barrier. (C) 1999
Wiley-Liss, Inc.