TUNING OF LIPID BILAYER FLUIDITY REGULATES MEDIATED ELECTRON-TRANSFERREACTIONS OF GLUCOSE-OXIDASE IMMOBILIZED ON LIPID BILAYER FILMS ON ANELECTRODE

A ferrocene with double long chains, 2C(16)GluFc, was synthesized and mediated electron transfer reactions of glucose oxidase, immobilized o n a lipid cast film containing the ferrocene, were examined. A basal p lane pyrolytic graphite (BPG) electrode modified with a cast of 2C(16) GluFc and a synthetic lipid, dihexadecyldimethylammonium bromide (2C(1 6)N(+)Br(-)) gave phase transition dependent cyclic voltammograms. Nor mal pulse voltammetry proved that the enhancement of the faradaic curr ent at the electrode at temperatures above the phase transition is asc ribable to the increase in the apparent diffusion rate constants. Gluc ose oxidase was immobilized on the cast film of 2C(16)GluFc/2C(16)N(+) Br(-) on BPG and ferrocene-mediated catalytic electron transfer reacti ons in the presence of glucose were explored. Temperature experiments have revealed that the tuning of the lipid bilayer fluidity between cr ystal-to-liquid crystalline phase regulates the catalytic electron tra nsfer reaction. (C) 1997 Elsevier Science S.A.