Introduction and establishment of apricot in vitro through regeneration ofshoots from meristem tips

Citation
O. Perez-tornero et al., Introduction and establishment of apricot in vitro through regeneration ofshoots from meristem tips, IN VITRO-PL, 35(3), 1999, pp. 249-253
Citations number
17
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
ISSN journal
10545476 → ACNP
Volume
35
Issue
3
Year of publication
1999
Pages
249 - 253
Database
ISI
SICI code
1054-5476(199905/06)35:3<249:IAEOAI>2.0.ZU;2-S
Abstract
In this study different aspects of the in vitro introduction and establishm ent of apricot cultivars were investigated through meristem tip culture. Th e best time to introduce the meristems of 'Canino' was when buds were start ing to swell. Various plant growth regulators were used at different concen trations on four distinct apricot cultivars to promote the development of t he meristems to shoots which could then be micropropagated. Very diverse re sults were obtained depending on the genotype. In general. meristems did no t survive without N-6-benzyladenine. Concentrations of gibberellic acid fro m 2 to 4 mg 1(-1) (5.8-11.4 mu M) promoted explant elongation. This step, w as critically important to obtain apricot shoots large enough to be transfe rred to proliferation medium.