The use of histology in the study of plant tissue culture systems - Some practical comments

Histological methods have contributed significantly to our understanding of in vitro culture systems. A good histological study based on anatomical an d histochemical changes provides insight into cellular processes and provid es clues that allow for the proposal of hypotheses for further experimentat ion. This article sen;es to draw attention to the use of a histological app roach to one's experimental system. Some of the common mistakes in the hand ling and processing of explants are discussed. A protocol for the plastic e mbedding method is detailed.