A convenient protocol for extraction and purification of DNA from Fragaria

Citation
Ja. Mercado et al., A convenient protocol for extraction and purification of DNA from Fragaria, IN VITRO-PL, 35(2), 1999, pp. 152-153
Citations number
15
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
ISSN journal
10545476 → ACNP
Volume
35
Issue
2
Year of publication
1999
Pages
152 - 153
Database
ISI
SICI code
1054-5476(199903/04)35:2<152:ACPFEA>2.0.ZU;2-6
Abstract
In this paper we describe a simple and efficient DNA extraction protocol fo r Fragaria species, highly recalcitrant genus due to the large amount of po lyphenols and polymeric carbohydrates present in strawberry tissues. The pr otocol yields a high quality DNA that can be amplified by polymerase chain reaction and digested with restriction endonucleases.