Isolation of a trypsin inhibitor with deletion of N-terminal pentapeptide from the seeds of Momordica cochinchinensis, the Chinese drug mubiezhi

A trypsin inhibitor, MCCTI-1, with a molecular weight of 3479 Da as determi ned by mass spectrometry, was isolated from Momordica cochinchinensis seeds with a procedure involving extraction with 5% acetic acid, ammonium sulfat e precipitation, ion exchange chromatography on CM-Sepharose and reverse-ph ase high performance liquid chromatography. The sequence of its first 13 N- terminal amino acid residues was ILKKCRRDSDCPG which was about 85% identica l with the sequence of trypsin inhibitor MCTI-1 from Momordica a charantia Linn, When compared with the sequences of most other squash family trypsin inhibitors, the sequence of MCCTI-1 was characterized by the deletion of a pentapeptide from the N-terminus. Trypsin inhibitors also existed in seeds of some hitherto uninvestigated Cucurbitaceae species. (C) 1999 Elsevier Sc ience Ltd. All rights reserved.