A new human synovial sarcoma cell line, HS-SY-3, with a truncated form of hybrid SYT/SSX1 gene

Recent cytogenetical and molecular studies have indicated that synovial sar coma harbors a t(X;18)(p11.2;q11.2) translocation, resulting in the formati on of a hybrid SYT/SSX(SSX1 or SSX2) gene. We newly established a human cel l line, HS-SY-3, from a synovial sarcoma. HS-SY-3 cells were shown to harbo r the pathognomonic t(X;18)(p11.2;q11.2) translocation by chromosome analys is but not to exhibit the classical hybrid SYT/SSX transcripts induced by t his translocation, using RT-PCR. To determine the reason for this discrepan cy, we analyzed cDNA from HS-SY-3 cells, as well as the original sarcoma ti ssue by the rapid amplification of cDNA 3' end assay, and found that the ch imaeric cDNA was 240 bp shorter than the previously established SYT/SSX1 cD NA due to truncation of the 3' side of SSX1. The HS-SY-3 cells should be us eful for future functional studies of the SYT/SSX chimeric gene. Int. J. Ca ncer 82:459-464, 1999. (C) 1999 Wiley-Liss, Inc.