Cytogenetic and CGH studies of four neuroendocrine tumors and tumor-derived cell lines of a patient with multiple endocrine neoplasia type 1

A malignant insulinoma (LOHG-I), a carcinoid of the lung (LOHG-L), a parath yroid adenoma (LOHG-NSA), and a fibroma (LOHG-F) were obtained from a patie nt with multiple endocrine neoplasia type 1 (MEN1). Long-term cultures were established. Essential neurobiological properties of the cell lines were p roven immunocytochemically and by electron microscopy. Molecular analysis o f the germline DNA showed a 4 bp deletion in exon 3 of the MEN1 gene. Cytog enetic and CGH analyses of the tumors/tumor cell lines revealed diploidy an d balanced and unbalanced structural aberrations different for each tumor. Chromosomes 6q21, 11q and 17q were most frequently involved in clonal struc tural aberrations.