HLA-B27 subtypes and HLA class II alleles in Japanese patients with anterior uveitis

PURPOSE. Some patients with anterior uveitis (AU) ankylosing spondylitis (A S) and are HLA-B27 class I-positive. The purpose of this study was to inves tigate whether there are differences in HLA at the allele level among each group of patients with AU. METHODS. Seventy-three patients with AU were studied. They were classified into three groups: 31 with AS-associated AU. 14 with HLA-B27-associated AU, and 28 with idiopathic AU. Three control groups without AU were used: 138 random subjects, 33 HLA-B27-positive healthy subjects, and 19 HLA-B27-posit ive patients with AS. DRB1 and DQB1 genotyping was performed using polymera se chain reaction (PCR)-single-strand conformation polymorphism (PCR-SSCP) and PCR-restriction fragment length polymorphism. HLA-B27 subtype was deter mined by PCR-SSCP. RESULTS. There was no difference in the frequency of any class I antigen ex cept HLA-B27 among the patients studied. The frequencies of HLA-DR12 in AS- associated AU and HLA-DR1 in HLA-B27-associated AU showed an increase. In H LA-R27-associated AU, DRB1*0101 and DQB1*0501 were increased compared with HLA-B27-positive control subjects. When HLA-B27 subtype distribution was co mpared among the groups, the proportion of B*2704 was significantly lower i n HLA-B27-associated AU (P = 0.037), however, such a difference was nut pre sent in AS-associated groups. CONCLUSIONS. These results indicated that B*2704 seemed to be less suscepti ble to AU compared with B*2705 in Japanese subjects. The increase of HLA-DR 12 and HLA-DR1 in AU may be caused by linkage disequilibrium with B*2704 an d B*2705, respectively.