Mp. Kelly et al., ACTIVITY AND EXPRESSION OF NA-H+ EXCHANGER ISOFORM-1 AND ISOFORM-3 INKIDNEY PROXIMAL TUBULES OF HYPERTENSIVE RATS(), Circulation research, 80(6), 1997, pp. 853-860
Increased activity of the cellular Na+-H+ exchangers (NHEs), especiall
y isoform 1 (NHE-1), is a recognized intermediate phenotype of hyperte
nsion. NHE activity has been demonstrated to be increased in proximal
tubules of the spontaneously hypertensive rat (SHR). However, with the
recent cloning of other members of this family of transporters, it is
unclear which isoforms may contribute to this increased activity. We
have used specific antibodies raised against glutathione-S-transferase
fusion proteins of rat NHE-1 and NHE-3 to determine the relative cont
ributions of these isoforms to the NHE activity in freshly isolated an
d cultured proximal tubule cells from SHR and Wistar-Kyoto (WKY) normo
tensive control rats. In freshly isolated proximal tubule cells, NHE a
ctivity was elevated almost 3-fold in SHR cells (P<.001), and in both
rat strains, the contribution from NHE-1 and NHE-3 was approximately e
qual. Western blots of membranes from these cells showed equal amounts
of NHE-1 protein in SHR and WKY cells. However, NHE-3 protein express
ion was increased 50% in SHR cells (P<.001), and this may account for
the elevated activity of this isoform in SHR. The effect of culturing
these cells in vitro was then examined. Although total NHE activity in
both cell types was decreased during culture, this was mainly due to
loss of expression of NHE-3 protein. NHE-1 activity was persistently e
levated in the SHR cells in culture. These findings suggest that eleva
ted NHE activity in SHR proximal tubules could be mediated by two mech
anisms: (1) increased NHE-1 activity without any increased NHE-1 prote
in content that persists despite culture and may resemble those change
s described for extrarenal tissues and (2) increased NHE-3 activity du
e to increased expression of NHE-3 protein. Disappearance of NHE-3 dur
ing culture implies that our culture conditions did not replicate the
in vivo environment and may have removed the factors contributing to t
he increased NHE-3 expression in SHR cells.