Super-high-speed liquid chromatography of proteins and peptides on non-porous Micra NPS-RP packings

The new generation of non-porous silica RP packings commercially available from Micra Scientific was tested for separations of peptides and proteins b y means of the gradient HPLC. Extremely high-speed separations were achieve d using conventional chromatographic equipment: six proteins could be compl etely separated within six seconds. Tryptic digest peptides could be resolv ed in more then 40 components within 2-3 min. The effect of the experimenta l parameters such as temperature, flow rate etc. was investigated. (C) 1999 Elsevier Science B.V. All rights reserved.