High-performance liquid chromatographic separation of novel atropic alpha,alpha-disubstituted-beta-amino acids, either on different beta-cyclodextrin-bonded phases or as their 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide derivatives

The high-performance liquid chromatographic enantioresolution of free and N - and/or C-protected derivatives of (R,S)-2',1':1,2; 1 ",2 ":3,4-dinaphthcy clohepta-1,3-diene-6-aminomethyl-6-carboxylic acid (beta(2)-Bin) by direct and indirect methods is reported. The direct separation was carried out on native and different derivatized beta-cyclodextrin-bonded phases. The indir ect resolution was achieved by applying pre-column derivatization with 1-fl uoro-2,4-dinitrophenyl-5-L-alanine amide. The effects of different paramete rs such as the mobile phase composition and the structures of the compounds on the enantiomeric resolution are discussed. (C) 1999 Elsevier Science B. V. All rights reserved.