High-performance liquid chromatographic separation of carbohydrates on stationary phases prepared from polystyrene-based resin and tertiary amines - Effect of chemical structure of anion-exchange sorbents

Two types of new anion-exchange stationary phases were readily prepared by the reaction of porous particles of chloromethylated styrene-divinylbenzene copolymer with various amines. The stationary phases M-n, where n is the l ength of alkyl chain, were obtained by the reaction with N,N-dimethylalkyla mines. The reaction with N,N,N',N'-tetra-methyldiaminoalkanes also afforded the stationary phases D-n with a terminal functional group, where n is the length of methylene bridge. The HPLC separations of monosaccharides, disac charides and oligosaccharides were successfully performed on these stationa ry phases using electrochemical detection with a Ni-Ti alloy working electr ode in alkaline eluents. The effects of chemical structures of stationary p hases on the separation of carbohydrates are described. (C) 1999 Elsevier S cience B.V. All rights reserved.